Dr. Patrick Hayes, Professor/Associate Head – Barley breeding and products at Oregon State University connected with Floragenex in late 2008 as an early access customer. After initial trials of RAD based sequencing in barley demonstrated the power of the technology, Dr. Hayes chose Floragenex as a research partner for improving the existing genetic map in the Oregon Wolfe Barleys (OWB), a well characterized research population with a robust genetic map.

Customer Goals

• Expand the current genetic map for OWB.
• Evaluate RAD based sequencing in comparison to other SNP development and genotyping systems.
• Obtain sequence information that can be used as a reference for future genomic studies.

SNP Discovery

Floragenex sequenced two parental lines to identify polymorphisms in the bi-parental mapping population. These sequenced based markers would serve as potential markers on a genetic map.

SNP Genotyping

To evaluate the effectiveness of RAD based markers, 93 doubled haploid progeny of OWB were genotyped using RAD based sequencing. From that sequence information Floragenex assayed over 400 new SNP markers that were then placed on an existing genetic map. This information will be used in future studies focused on identifying important regions of the barley genome.