Discovery

Identify genetic variation in any species

Discovery efforts are an ideal strategy for identifying genetic variants and then translating those variants into traditional array, bead or PCR based genotyping assays.

Floragenex uses RAD sequencing (RAD-Seq) and double digest RAD sequencing (SBG / ddRAD-Seq) as strategies to identify single nucleotide polymorphisms (SNPs). For detecting microsatellite (SSRs) variants, Floragenex offers Geno Sat® technology in collaboration with our partners at GenoScreen. 

Why select a Discovery project?

For almost three decades, scientists have been identifying genetic variants as tools to help answer fundamental questions in the life sciences. 

Variants identified from Floragenex Discovery next-generation DNA sequencing projects have been used and validated across a broad range of genotyping platforms, including BeadXpress™, Infinium™, iPlex™, KaSP™, TaqMan™ and more. In addition, all Discovery projects benefit from premium laboratory support, along with world-class bioinformatics analytics and reporting.


RAD-Seq & ddRAD-Seq: for SNP Discovery

  • Ideal for identifying from 500 to 100,000 single nucleotide variants
  • Can be applied in species with or without genomic resources and reference genome
  • Effective in complex genomes with repetitive structure
  • Identified SNPs provided with flanking genome sequence for easy translation into downstream genotyping assays

Geno Sat: for SSR Development

  • The best option for obtaining 2000+ sequences including microsatellite motifs
  • Can be applied in species with or without genomic resources and reference genome
  • Successfully applied in over 400 species across the world
  • Tightly integrated with next generation DNA sequencing technologies
  • Offered in partnership with GenoScreen

DISCOVERY PROJECTS INCLUDE:

  • Dedicated client support
  • Sample processing in batches of 95
  • Industry leading QA/QC procedures
  • Optimized RAD or Geno Sat library preparation
  • Illumina HiSeq 2000/2500 or Roche 454 Sequencing
  • Floragenex Sequence Analytics (for RAD-Seq):
    • Next Gen Sequencing quality control
    • Index / Barcode demultiplexing
    • De novo assembly (if requested)
    • Alignment
    • Variant calling
  • Microsatellite Bioinformatic Analysis (for Geno Sat)
    • Polymorphism evaluation on selected microsatellites (for Geno SAT)

  • 12 to 16 week turn around time
  • Data storage for up to 1 year