SBG / ddRAD Sequencing

Discovery and Genotyping using next gen DNA sequencing

Sequence Based Genotyping (SBG), also known as double digest RAD Sequencing (ddRAD-Seq), is a fractional genome sequencing strategy, designed to efficiently identify and score genetic variants across any genome.  SBG technology involves digesting a target genome with two restriction nucleases and then surveying the resulting fragments for SNP variants using next generation DNA sequencing. With SBG technology, development of hundreds to tens of thousands of genetic markers can be accomplished quickly and effectively.

 

SBG / ddRAD Genotyping Applications

PhylogenETIC ANALYSIS

Population Structure

Linkage and QTL Mapping

Association MAPPING

SBG and ddRAD-Seq are routinely used for a range of molecular genetic applications

  • Identification of genetic variants (SNPs)
  • Germplasm assessment
  • Population genetics
  • Genome wide association studies (GWAS)
  • Linkage and QTL mapping
  • Marker-trait association
 

How does SBG work?

  • SBG works by first fragmenting the target genome using both a common and rare digesting restriction enzyme. After whole genome digestion, a series of molecular processing steps transform the DNA into a fragment library suitable for sequencing on a NGS platform.
  • Depending on the scientific goal, either single end or paired end sequencing can be used to generate the appropriate amount of genomic information and markers. 
  • Sequence data can then analyzed to identify and score genetic variations in the samples or population of interest. 

Sequence Based Genotyping technology is provided under license from KeyGene, N.V. which owns patents and patent applications protecting its Sequence Based Genotyping technologies.